Staining of Yersinia

Yersinia pestis: Properties, Disease, Lab Diagnosis

Wayson or Giemsa staining demonstrates the typical bipolar or safety pin appearance. Two ends are darkly stained with a clear central area. All Yersinia pestis may stain as bipolar cells, but all bipolar-staining cells are not Y. pestis. Culture. Y. pestis is a member of the Enterobacteriaceae family. It is aerobic and facultatively anaerobic Gram Staining Reaction - Yersinia pestis is a Gram -ve (Negative) bacterium. In the microscopic view of Giemsa or Methylene blue-stained smear, shows bipolar staining (safety pin-like appearance) with the two ends densely stained and a clear central area

Morphology & Culture Characteristics of Yersinia pesti

  1. Yersinia spp. are members of the Enterobacteriaceae. They are short, pleomorphic Gram-negative rods or coccobacilli, which often exhibit bipolar staining. Yersinia pestis is nonmotile. Other species are nonmotile at 98.6°F (37°C) but motile at temperatures less than 86°F (30°C) by means of peritrichous flagella
  2. Bipolar staining is typically described as characteristic of Yersinia pestis; however, it is important to note that most Enterobacteriaceae can have a bipolar staining appearance when found in clinical specimens. The patient improved clinically on empiric therapy with vancomycin and ceftazidime, and she was switched to meropenem
  3. Yersinia is a genus of bacteria characterized as gram-negative bacilli that are facultative anaerobic with bipolar staining. There are 2 enteropathogenic species that cause yersiniosis, Y. enterocolitica and Y. pseudotuberculosis. Infections are manifested as pseudoappendicitis or mesenteric lymphadenitis, and enterocolitis
  4. It is Gram-negative and, when stained with Wayson's stain or methylene blue, it shows typical bipolar staining which is an important feature of metachromatic granules. It is highly pleomorphic, non-motile and non-sporing. 2. Cultural Characteristics of Yersinia Pestis
  5. Yersinia pestis is a Gram-negative, coccobacilli, about (1.5 X 0.7) mm in size, arranged singly in short chains or in small groups. When stained with Giemsa or methylene blue, it shows bipolar staining with two ends densely stained and the central area clear
  6. Properties (Yersinia pestis) Capsule: Positive (+ve) Catalase: Positive (+ve) Citrate: Negative (-ve) Flagella: Non-Flagellated: Gas: Negative (-ve) Gelatin Hydrolysis: Negative (-ve) Gram Staining: Negative (-ve) Growth in KCN: Negative (-ve) H2S: Negative (-ve) Hemolysis: Negative (-ve) Indole: Negative (-ve) Motility: Non-Motile: MR (Methyl Red) Positive (+ve) Nitrate Reductio
  7. Differential staining: Gram positive organisms: Streptococcus sp. , Enterococcus sp. , Listeria sp. , Bacillus sp. etc. Gram negative organisms: Pseudomonas sp. , Salmonella sp. , Klebsiella sp. , Yersinia sp. etc. Acid fast organisms: Mycobacterium sp. Non acid fast organisms: Enterobacter sp. Special staining: Capsule stain positive bacteria

Yersinia pestis — Gram Stain. Yersinia pestis. — Gram Stain. Gram-negative bacilli (0.5 to 0.8 by 1 to 3 microns), single or short chained. Sometimes bipolar staining (closed safety pin). Note: The safety pin appearance is best observed in direct smears of infected specimens. All bipolar staining cells are not Yersinia pestis Gram stain of cytospin-concentrated CSF showed many neutrophils and intracellular gram-negative rods with bipolar staining. The organism was identified as Enterobacter aerogenes.Bipolar staining is typically described as characteristic of Yersinia pestis; however, it is important to note that most Enterobacteriaceae can have a bipolar staining appearance when found in clinical specimens Yersinia are gram-negative bacteria and are described as facultative anaerobes, which means that they are capable of surviving in both aerobic and anaerobic environments. Though several species are motile below 37 °C (98.6 °F), all Yersinia organisms are rendered nonmotile at this temperature and above Yersinia pestis is a nonmotile, slow-growing, facultative organism classified in the family Enterobacteriaceae. It appears as plump, gram-negative coccobacilli that are seen mostly as single cells or pairs, which may exhibit bipolar staining from a direct specimen if stained with Wright's or Giemsa stains. This appearance has been referred to as safety pin-like

Yersinia - an overview ScienceDirect Topic

  1. ation of Gram, Wright, Giemsa, or Wayson's stained smears of peripheral blood, sputum, or lymph node specimen.Visualization of bipolar-staining, ovoid, Gram-negative organisms with a safety pin appearance permits a rapid presumptive diagnosis of plague
  2. It was due to antibodies, mostly of the IgG class, occurring in low titre, which react with intracytoplasmic antigens in thyroid, as staining of live thyroid epithelial cells was negative. The pattern of immunofluorescence on thyroid sections caused by these antibodies could not be distinguished from that caused by smooth muscle antibodies by appearance only
  3. The Wayson stain is a basic fuchsin-methylene blue, ethyl alcohol-phenol microscopic staining procedure. It was originally a modified methylene blue stain used for diagnosing bubonic plague . [1] With this stain, Yersinia pestis appears purple with a characteristic safety-pin appearance, [2] which is due to the presence of a central vacuole

Yersinia pestis is a nonmotile, slow-growing, facultative organism classified in the family Enterobacteriaceae. It appears as plump, gram-negative coccobacilli that are seen mostly as single cells or pairs, which may exhibit bipolar staining from a direct specimen if stained with Wright stains. This appearance has bee Yersinia pestis Major Characteristics of Yersinia pestis Gram stain Morphology: Gram-Negative rods Colony Morphology: At 24 hours - grey-white, translucent colonies, usually too small to be seen as individual colonies. At 48 hours - colonies growing on SBA are gray white to slightly yellow and opaque. Older cultures may have Frie Bipolar staining largely features in bacterial infections, such as yersinia pestis. The most familiar example of this infection is bubonic plague. Also, meningitis occurs in 10 percent of people infected. Symptoms include fever, stiff neck, headache and comas. Burkholderia mallei features bipolar staining as well General characteristics Y. pestis is a nonmotile, stick-shaped, facultative anaerobic bacterium with bipolar staining (giving it a safety pin appearance) that produces an antiphagocytic slime layer. Similar to other Yersinia species, it tests negative for urease, lactose fermentation, and indole Wayson stain showing the characteristic safety pin appearance of Yersinia pestis, the plague bacillus. Image courtesy of Centers for Disease Control and Prevention (CDC), Atlanta, Ga...

Bipolar staining of gram-negative bacillus in

Yersinia pestis is a nonmotile, slow-growing, facultative organism classified in the family Enterobacteriaceae. It appears as plump, gram-negative coccobacilli that are seen mostly as single cells or pairs, and which may exhibit bipolar staining from a direct specimen. B. Histor Yersinia ruckeri is the causative agent of enteric redmouth disease. It is a gram-negative rod bacteria of the family enterobacteriaceae. It was first isolated from rainbow trout ( Onchorhyncus mykiss) in the 1950s by Rucker ( Ross et al., 1966 ). Enteric redmouth is a subacute to acute systemic infection Gram Stain.Gram-negative rods (0.5 - 0.8 x 1- 3 μm).Giemsa stain: Bipolar staining Gram stain: Bipolar staining may be poor Additional Information. Can be misidentified as: Shigella spp., H 2 S(-) Salmonella spp., Acinetobacter spp., or Yersinia pseudotuberculosis by automated ID systems . Biosafety Level 2 agent (Use BSL3 for large volume. ( http://www.abnova.com ) - Wright and Giemsa stains are used to stain peripheral blood and bone marrow smears. The components are oxidized eosin Y, methylen..

Enterobacteriaceae: Yersinia spp

E.C.D. Todd, in Encyclopedia of Food Safety, 2014 Background. The genus Yersinia belongs to the Enterobacteriaceae family, and of the 11 species, only 3 are known to be pathogenic to humans, the plague bacillus, Yersinia pestis, and two that cause gastroenteritis: Yersinia enterocolitica and Yersinia pseudotuberculosis. Actually, Y. pestis is more genetically related to Y. pseudotuberculosis. INTRODUCTION TO ALBERT STAINING. Corynebacterium is the genus of Gram +ve, non-acid-fast, non-sporing, non-motile bacilli. The most important member of the genus is Corynebacterium diphtheriae, the causative agent of Diphtheria disease in children.The C. diptheriae or diphtheria bacillus was first described by Klebs but Loffler was the first to cultivate it in the laboratory and also called as. Yersinia pestis is a small oval, Gram-negative, capsulate coccobacillus with a characteristic bipolar staining phenotype (occurring at either end of the bacillus). Y.pestis is thought to have evolved over thousands of years from the ancestral species Y.pseudotuberculosis, to become a flea-vectored pathogen and in the process has acquired. Yersinia Are short, pleomorphic gram negative rods that can exhibit bipolar staining Are catalase positive, oxidase negative and microaerophilic or facultative Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising Definition. The Yersinia pestis bacterium is associated with the disease known as plague. The genus Yersinia is a member of the enterobacteria family and includes three human pathogens. One of these - Yersinia pestis - is a gram-negative, non-motile, non-spore-forming coccobacillus. It can grow in a wide range of temperatures and depends on other animals in order to pass on to humans.

Yersinia pestis. Gram-negative, non-motile . Non-spore-forming coccobacillus. Facultative anaerobe, unencapsulated. Bipolar staining (looks like safety pin) Causative agent of Black Plague. LD. 50. ranges from 1 to 108 depending on strainPathogenicity linked with plasmids pCD1, pPCP1, and pMT1 The Wright stain often reveals the bipolar staining characteristics of Y. pestis, whereas the Gram stain may not. The Wright-Giemsa stains are the most reliable for accurately highlighting the bipolar staining characteristics of these gram-negative rods (Fig. A2). b. Wayson stain, another polychromatic stain, can be used instead of Wright. Y. pestis causes plague and is transmitted by fleas. The most common clinical manifestation is acute febrile lymphadenitis, called bubonic plague. Less common forms include septicemia, pneumonia, pharyngeal and meningeal plague. The epidemiology, microbiology and pathogenesis of Y. pestis will be reviewed here When we say yersinia pestis has a polar stain what do we mean? The organism stains quickly at the poles. The bipolar stains in yersina makes it look like _____ or _____ because of the stain some people have referred to them as _____ Stain like cocci or pair of cocc Synovial specimens from 13 patients with Yersinia enterocolitica O:3-triggered reactive arthritis and from 16 control patients were studied using polymerase chain reaction and immunocytochemical staining techniques. Results. Yersinia chromosomal DNA was not found in any of the synovial specimens from Yersinia-triggered arthritis patients or.

Dark stained bipolar ends of Yersinia pestis can clearly be seen in this Wright's stain of blood from a plague victim, 1993. The actual cause of the... Mycobacterium tuberculosis in a sputum smear is stained using fluorescent auramine with acridine orange counterstain, 1971 Changes in mucosal architecture and detection of Yersinia enterocolitica in distal mouse colon 2 days after infection. A, Hematoxylin-eosin staining revealed crypt lengths to be focally increased in infected monolayers (arrows). B, Quantification of crypt length (P < .05; 15 crypts in 5 mice each) 5) All of the statements given below about the Yersinia enterocolitica is correct, EXCEPT a) The bacteria are gram-negative, non-motile b) Consist of bipolar staining property c) The most common serotypes causing human gastroenteritis is O:1 d) Transmitted to humans may occur via contaminated food and wate The bacterium stains Gram-negative and cultures can grow at body temperatures of 95 to 98.6 Fahrenheit. Yersinia pestis is the only species of its genus that remains immotile at room temperature. The bipolar staining is more commonly observed in smears made from clinical specimens rather than cultures. Bipolar Staining in Burkholderia Pseudomalle

Yersinia Pestis in Humans Systematic Bacteriolog

Exam III - Medical Microbiology 3200 with Baldwin at

Yersinia infection is a notifiable condition. Patients with signs of pneumonic plague should be isolated immediately and placed on droplet precautions. Diagnosis of plague may be suggested by characteristic clinical findings together with a history of potential exposure in an endemic area. Yersin.. Staining - Y. pestis is a small (1 x 0.5µm) Gram-negative bipolar staining rod. A presumptive diagnosis of plague may be made if the direct stain made from a bubo, blood, or tracheal or lung aspirates from a patient with compatible clinical symptoms shows small bipolar staining rods Figure: Scanning electron microscope image of Yersinia pestis. Source: www.mirror.co.u Yersinia ist der Name einer Gattung von gramnegativen, meist stäbchenförmigen Bakterien aus der Familie der Yersiniaceae, welche sich unter fakultativ aneroben Bedingungen vermehren. Im Plural werden Vertreter der Gattung eingedeutscht auch als Yersinien bezeichnet. Nach dem Code der Nomenklatur der Bakterien ist das grammatische Geschlecht des Gattungsnamens feminin A. Yersinia pestis is a nonmotile, slow-growing, facultative organism classified in the family Enterobacteriaceae. It appears as plump, gram-negative coccobacilli that are seen mostly as single cells or pairs, which may exhibit bipolar staining from a direct specimen if stained with Wright stains

Yersinia pestis- characteristics, habitat and virulence

  1. Yersinia pestis IFA staining of the F1 capsule The Yersinia research team The Yersinia research group is conducting basic and applied research to study the plague pathogen Yersinia pestis. Our research focuses on studying host-microbe interactions, mechanisms of pathogenesis and mechanisms of innate and adaptive immunity that could be used for.
  2. Plague bacteria (Yersinia pestis),computer illustration.Y.pestis are gram-negative bacteria from Enterobacteriaceae family,they have ovoid shape bipolar staining (more intensively stained at poles).The bacterium causes bubonic plague (the Black Death of Middle Ages).The bacterium is primaril
  3. al pain, and diarrhea... Prairie dog died due to a Yersinia pestis plague infection, 1972
  4. Yersinia enterocolitica invasin triggers phagocytosis via beta1 integrins, CDC42Hs and WASp in macrophages. By Agnès Wiedemann. Cytotoxic Necrotizing Factor-Y Boosts Yersinia Effector Translocation by Activating Rac Protein. By Klemens Rottner and Martin Aepfelbacher
  5. Gram negative rod-ovoid 0.5-0.8 µm in width and 1-3 µm in length (safety pin appearance), bipolar staining (Giemsa) facultative intracellular, non-motile. Disease/Infection. Y. pestis causes a zoonotic disease of rodents and in humans can take the form of bubonic, septicemic or pneumonic plague. Pathogenicit

Biochemical Test and Identification of Yersinia pesti

INTRODUCTION. In the genus Yersinia, three species are important human pathogens: Yersinia pestis, Yersinia enterocolitica, and Yersinia pseudotuberculosis.The yersinioses are zoonotic infections of domestic and wild animals; humans are considered incidental hosts that do not contribute to the natural disease cycle Photomicrograph of Yersinia enterocolitica using a Flagella-staining technique, 1980. Symptoms of yersiniosis are fever, abdominal pain, and diarrhea... The plague is caused by infection with Yersinia Pestis, carried by fleas that infest rodents which then bite humans 134 Yersinia Pestis Bilder und Fotos. Durchstöbern Sie 134 yersinia pestis Stock-Fotografie und Bilder. Oder starten Sie eine neue Suche, um noch mehr Stock-Fotografie und Bilder zu entdecken. Bubonic plague smear, prepared from a lymph removed from an adenopathic lymph node, or bubo, of a plague patient, demonstrates the presence of the..

Video: What is Staining? Definition, Objectives, Mechanism

Yersinia gram stain - SD Dept

• Stains well; bipolar safety pin stain may occasionally be seen with Wright or Giemsa stain, however, this is not reliable; hard to see on Gram stain B. pseudomallei Yersinia pestis, Gram stain, 1000x (ASM) Yersinia pestis, Wright stain, Bipolar staining, 1000x REFER TO Burkholderia pseudomallei Tab (CDC) REFER TO Yersinia pestis Ta Romanowsky Stains are the stains that are used in hematology and cytological studies, to differentiate cells in microscopic examinations of blood and bone marrow samples. These stains are also applied to detect the presence of parasites in the blood such as malaria parasites. There are various Romanowsky staining types that apply the same. Yersinia enterocolitica is a more common manifestation of yersinosis, and causes abdominal pain, fever, It has a particularly thin layer of peptidoglycan that causes it to form bipolar staining when Gram stained. Bacteroides fragilis is a common normal flora agent of the gastrointestinal tract Interestingly, with a careful staining procedure, Yersinia cells stain very heavily on each end and take up very little stain at the center, giving the cells a unique 'safety pin' appearance.Yersinia pestis is a natural pathogen of rats. Most rats that carry the bacteria develop plague and die very quickly

We offer key QC assay formats relevant to your needs. Our analysis capabilities include: Molecular - qPCR, PCR, western blotting, Southern blotting, electrophoresis, nuclease contamination, bacterial growth, cell component isolation kits (DNA, RNA, protein) Immunological - ELISA, western blotting, Ouchterlony double diffusion, cell staining, histology, serolog Yersinia pestis: The cause of human plague, Yersinia pestis may be identified microscopically by examination of Gram, Wright, Giemsa, or Wayson's stained smears of peripheral blood, sputum, or lymph node specimen. Visualization of bipolar-staining, ovoid, Gram-negative organisms with a safety pin appearance permits a rapid presumptive diagnosis of plague Yersinia (formerly Pasteurella) pestis is a short bacillus that often shows bipolar staining (especially with Giemsa stain) and may resemble a safety pin. Massive human epidemics (eg, the Black Death of the Middle Ages, an epidemic in Manchuria in 1911) have occurred

Bubonic plague as deadly as the Black Death &#39;could return

The genus Yersinia is composed of Gram negative, bipolar staining coccobacilli. Like other Enterobacteriaceae, their metabolism is fermentative. Y. pestis produces a thick anti-phagocytic slime layer, while Y. enterocolitica is motile at 28° Yersinia enterocolitica and Y. pestis can be differentiated on the basis of which of the following tests? Solve it.... 9. Which of the following(s) bacteria belong to the family Entero-bacteriaceae? Solve it.... 10. Xylose lysine deoxycholate (XLD) agar is better selective medium than the deoxycholate citrate agar (DCA) because Solve it...

In Microbiology, Giemsa stain is used for staining inclusion bodies in Chlamydia trachomatis, Borrelia species, and if Wayson's stain is not available, to stain Yersinia pestis. Giemsa stain also is used to stain Histoplasma capsulatum , Pneumocystis jiroveci , Klebsiella granulomatis, Talaromyces marneffei (formerly called Penicillium. In humans, Yersinia pestis causes plague and Yersinia enterocolitica causes yersiniosis. Infection with Yersinia pseudotuberculosis is uncommon and causes similar symptoms to yersiniosis.. The plague bacillus Y pestis is transmitted to people mainly by the bites of infected fleas. Infection is characterised by the sudden onset of systemic symptoms such as fever and painful swelling of lymph. Yersinia enterocolitica is a pleomorphic, gram-negative bacillus that belongs to the family Enterobacteriaceae. As a human pathogen, Y enterocolitica is most frequently associated with enterocolitis, acute diarrhea, terminal ileitis, mesenteric lymphadenitis, and pseudoappendicitis, [] with the spectrum of disease ranging from asymptomatic to life-threatening sepsis, especially in infants Pathogenic Yersinia species employ type III machines to target effector Yops into the cytosol of eukaryotic cells. Yersinia tyeA mutants are thought to be defective in the targeting of YopE and YopH without affecting the injection of YopM, YopN, YopO, YopP, and YopT into the cytosol of eukaryotic cells. One model suggests that TyeA may form a tether between YopN (LcrE) and YopD on the.

Yersinia species grow on blood agar and MacConkey agar at room temperature. Y. pseudotuberculosis and Y. enerocolica are motile, unlike Y. pestis; Biochemical tests to identify particular species; Specific fluorescent antibody staining of lymph node aspirates to identify Y. pestis; Contro 6. Which of the following bacteria will stain purple color after Gram staining? A) Bacillus subtilis. B) E coli. C) Yersinia pestis. D) Pseudomonas aeruginosa. 7. The incorrect pair of food borne illness and its causative microorganism is . A) brucellosis - Brucella sp. B) Peptic ulcers - Bacillus subtitles. C) Bubonic plague - Yersinia.

Albert Stain: Principle, Procedure and Results - LearnMicro at Indiana University School of Medicine - StudyBlueFree picture: bubonic, plague, smear, demonstratingFree picture: yersinia pestis, direct, fluorescentEnterobacteria: Escherichia, Salmonella, Shigella

Yersinia is a genus of Gram-negative, rod-shaped, facultative anaerobes within the family Enterobacteriaceae.Yersinia comprises several pathogenic species, which cause diseases in humans and other animals, including fish.Yersinia ruckeri is the causative agent of enteric redmouth disease in various species of salmonids worldwide. It was described from rainbow trout in the Hagerman Valley of. Yersinia pestis is a zoonotic pathogen that is most commonly transmitted through fleas that feed on infected rodents. Y. pestis is a Gram-negative, non-motile, non-spore-forming coccobacillus that is also a facultative anaerobe. [3] In the past, this pathogen ravaged cities throughout Europe, Asia, and Africa, takin thousands of lives with. Yersinia strains. A strain of Yersinia enterocolitica type IX was kindly donated by Dr P. Ahvonen, of the Municipal Bacteriological Laboratory, Helskini, Finland, as a freeze-dried culture. On examination it conformed to the biochemical and cultural characters described by Mollare & Chevaliet r (1964) and in these respect Giemsa stain is a type of Romanowsky stain, named after Gustav Giemsa, a German chemist who created a dye solution. It was primarily designed for the demonstration of malarial parasites in blood smears, but it is also employed in histology for routine examination of blood smear. Principle: Giemsa stain is a differential stain and contains a mixture of Azure, Methylene blue, and Eosin dye. It.